DNA Fingerprinting Using Restriction Enzymes, Group Size for 8 Gels

Teach your students about restriction enzyme digests in the context of forensic science! Your students will cut DNA with restriction enzymes and then compare the banding pattern of the crime scene DNA versus that of two suspects using agarose gel electrophoresis.Group Size:For 8 gelsTime Required:Complete in 1 hour 20 minutes to 1 hour 45 minutesKit Includes:Instructions, “crime scene” and "suspect" Ready-to-Load DNA samples, DNA Standard Marker, Dryzymes - Eco RI and Hind III, Enzyme Reaction Buffer, Reconstitution Buffer, UltraSpec-Agarose, 10X Gel Loading Solution, 50X Concentrated Electrophoresis Buffer, Practice Gel Loading Solution, SYBR Safe stain, FlashBlue DNA Stain, 1 mL pipet, Microtipped Transfer Pipets, & Microcentrifuge Tubes.All You Need:DNA Electrophoresis, Micropipettes: 5-50 µl, Water Bath, Blue Light Visualization System (use if staining with SYBR Safe) White Light Box (use if staining with FlashBlue), & Microwave or Hot plate.Storage:Some Components Require Freezer Storage Upon Receipt

• Kit Includes: Instructions, “crime scene” and "suspect" Ready-to-Load DNA samples, DNA Standard Marker, Dryzymes - Eco RI and Hind III, Enzyme Reaction Buffer, Reconstitution Buffer, UltraSpec-Agarose, 10X Gel Loading Solution, 50X Concentrated Electrophoresis Buffer, Practice Gel Loading Solution, SYBR Safe stain, FlashBlue DNA Stain, 1 mL pipet, Microtipped Transfer Pipets, & Microcentrifuge Tubes.
• Learn the biology, techniques, and applications of DNA fingerprinting
• Digest DNA from two suspects using the enzymes EcoRI and HindIII
• Separate and size DNA samples using gel electrophoresis
• Compare suspect and crime scene samples to determine ‘whodunnit’